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KMID : 0376419840080010189
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Chonbuk University Medical Journal
1984 Volume.8 No. 1 p.189 ~ p.200
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Experimental Study on the Mechanism of the Giant-Cells Formation
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Abstract
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This experiment was designed to investigate the mechanism of giant-cells formation. Methyl-cellulose(5mg/long, B.W., 3 times/week) was injected intraperitoneally during 1 week, 2 weeks, 3 weeks, and 4 weeks, respectively. Histological(hematoxylin-eosin, periodic acid-Schiff, and reticulin stain) and enzyme histochemical(acid phosphatase, alkaline phosphatase, and nonspecific esterase stain) findings were observed and the following results, were obtained.
1. After methyl-cellulose treatment, giant-cells appeared in spleen of 2 weeks group, in liver of 3 weeks group, and in lung of 4 weeks group, respectively. And the number of giant cells was increased in proportion to the duration of methyl-cellulose treatment.
2. Giant cells appeared generally on the hepatic sinusoidal wall, splenic red pulp, and alveolar septum. And perivascular lymphocytic infiltration was shown with proliferation of the reticuloendothelial cells such as macrophags.
3. Periodic acid Schiff stain showed strongly positive reaction with striking carbon phagocytosis in all giant cells.
4. Increased acid phosphatase and nonspecific esterase activities were observed in most giant-cells, and these reaction was more marked in proportion to the duration of methyl-cellulose treatment.
These observations permit the conclusion that giant-cells are formed by fusion of macrophages which migrated or proliferated by enhancement of cell-mediated immunity.
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KEYWORD
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